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cd34 cell fractions  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd34 cell fractions
    Cd34 Cell Fractions, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd34+cell+fractions/CD34-VioBlue/us12203096-489-17-31
    Average 99 stars, based on 4 article reviews
    cd34 cell fractions - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Physioxia enhances T-cell development ex vivo from human hematopoietic stem and progenitor cells
    Article Snippet: Mononuclear fractions were separated from human CB (CordUse, Orlando, Florida, and Cleveland Cord Blood Center, Cleveland, Ohio) by Ficoll Paque Plus (GE Healthcare, Piscataway, New Jersey) density gradient centrifugation. .. CD34+ cell fractions were further isolated through a human CD34 MicroBead Kit (Miltenyi Biotec, San Diego, California). .. These CB CD34+ cells were purified further by fluorescence-activated cell sorting (FACS; up to 99% CD34+ cell purity) using FACS Aria or FACS Fusion (Becton Dickinson, Erembodegrem, Belgium), or passing through separation column once more.

    Article Title: Preparation, expansion, and uses of adult pluripotent stem cells
    Article Snippet: .. Two weeks after co-culture of the isolated dormant tiny cells in the coculture system as described above, CD34+ cell fractions were enriched by MACS following the protocol recommended by the manufacturer (Miltenyi Biotec Inc. San Diego, CA). .. Briefly, trypsinized cells were incubated with an anti-CD34 (rat) antibody for 30 min, followed incubation for 15 min at 4° C. with anti-rat Microbeads.

    Article Title: Umbilical cord blood CD34(+)cell-derived progeny produces human leukocyte antigen-G molecules with immuno-modulatory functions.
    Article Snippet: Human umbilical cord blood units (UCBs) are an alternative source in allogeneic-stem-cell transplantation.. Human leukocyte antigen (HLA)–G is a tolerogenic molecule with a possible implication in UCB immunoregulatory effect.. HLA-G expression was observed in UCB myeloid and plasmacytoid dendritic cells; in contrast, CD34 cells did not produce thismolecule.

    other:

    Article Title: Purification of Human CD34 + CD90 + HSCs Reduces Target Cell Population and Improves Lentiviral Transduction for Gene Therapy
    Article Snippet: Human CD34 + cells from steady-state BM and G-CSF-mobilized leukapheresis collections were harvested and enriched as previously described., Enrichment of CD34 + cell fractions was performed according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Staining:

    Article Title: TALEN-mediated intron editing of HSPCs enables transgene expression restricted to the myeloid lineage.
    Article Snippet: CD34+ HSPCs were isolated from the mobilized LeukoPaks using a CliniMACS Plus Instrument, CliniMACS CD34 GMP MicroBeads, and CliniMACS Tubing Set LS (Miltenyi Biotec, Bergisch Gladback, Germany). .. CD34+ and CD34 cell fractions were stained with CD34-PE (Clone: REA1164; Miltenyi) and CD45-APC (Clone REA747;Miltenyi). .. Sample purity was assessed on a NovoCyte Penteon Flow Cytometer (Agi- lent Technologies, Santa Clara, CA, USA).

    Article Title: TALEN-mediated intron editing of HSPCs enables transgene expression restricted to the myeloid lineage
    Article Snippet: CD34+ HSPCs were isolated from the mobilized LeukoPaks using a CliniMACS Plus Instrument, CliniMACS CD34 GMP MicroBeads, and CliniMACS Tubing Set LS (Miltenyi Biotec, Bergisch Gladback, Germany). .. CD34+ and CD34- cell fractions were stained with CD34-PE (Clone: REA1164; Miltenyi) and CD45-APC (Clone REA747; Miltenyi). .. Sample purity was assessed on a NovoCyte Penteon Flow Cytometer (Agilent Technologies, Santa Clara, CA, USA).

    Magnetic Cell Separation:

    Article Title: Preparation, expansion, and uses of adult pluripotent stem cells
    Article Snippet: .. Two weeks after co-culture of the isolated dormant tiny cells in the coculture system as described above, CD34+ cell fractions were enriched by MACS following the protocol recommended by the manufacturer (Miltenyi Biotec Inc. San Diego, CA). .. Briefly, trypsinized cells were incubated with an anti-CD34 (rat) antibody for 30 min, followed incubation for 15 min at 4° C. with anti-rat Microbeads.

    Immunomagnetic Separation:

    Article Title: Umbilical cord blood CD34(+)cell-derived progeny produces human leukocyte antigen-G molecules with immuno-modulatory functions.
    Article Snippet: Human umbilical cord blood units (UCBs) are an alternative source in allogeneic-stem-cell transplantation.. Human leukocyte antigen (HLA)–G is a tolerogenic molecule with a possible implication in UCB immunoregulatory effect.. HLA-G expression was observed in UCB myeloid and plasmacytoid dendritic cells; in contrast, CD34 cells did not produce thismolecule.



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    Image Search Results


    FDA/EMA approved gene therapy product for rare monogenic diseases

    Journal: Clinical and Experimental Pediatrics

    Article Title: Development of orphan drugs for rare diseases

    doi: 10.3345/cep.2023.00535

    Figure Lengend Snippet: FDA/EMA approved gene therapy product for rare monogenic diseases

    Article Snippet: For instance, an autologous CD34+-enriched cell fraction, Strimvelis (GlaxoSmithKline plk, London, UK), which contains CD34+ cells transduced with a retroviral vector that encodes the human ADA cDNA sequence for ADA deficiency (introduced in 2016), and voretigene neparvovec-rzyl (Luxturna, Spark Therapeutics, Philadelphia, USA), for retinal dystrophy caused by mutations in the RPE65 gene, were approved by the FDA in 2017 at a cost of $850,000 per eye [ ].

    Techniques: Plasmid Preparation, In Vivo, Ex Vivo, Mutagenesis, Modification, Variant Assay